摘要
利用RAPD分子标记技术对14份来自内蒙古中东部草原的贝加尔针茅(StipabaicalensisRoshev.)、大针茅(S.grandisP.Smirn.)和克氏针茅(S.kryloviiRoshev.)进行遗传多态性分析。筛选出16个引物,扩增出223条DNA片段,其中多态性片段为184条,多态性比率为82.51%。结果表明:14份材料呈现出明显的种间相似性,同一种针茅首先聚在一起;每一种针茅都获得了其特有的RAPD条带。
Stipa baicalensis Roshev. , S. grandis P. Smirn, and S. krylovii Roshev are constructive species of the Inner Mongolia steppes. They are not only the main resources of forage in Inner Mongolia, but also the natural ecological defense of the Beijing-Tianjin area, boasting very important economical and environmental values. Studies on these Stipa species have been reported both in the domestic and overseas journals, focusing mainly on the grasses geographical distribution, and their biological and physiological characteristics. With the development of molecular biology techniques, studies on the molecular biology of Stipa species have drawn the attention of many researchers. Though analysis of the genetic similarities and genetic structure of one species of Stipa have been documented, research work on the genetic relationship between different Stipa species are still lacking. In the present study, 14 populations of three Stipa species from the middle and eastern Inner Mongolia steppes were analyzed with RAPD markers. We obtained 223 bands from the 16 primers, in which 184 bands were polymorphic. The rate of polymorphism was 82. 51%. There were 14 samples to show the obvious similarity among the species, and populations within the same species were initially clustered together. We obtained the special RAPD markers within the populations of the same species.
出处
《草地学报》
CAS
CSCD
2006年第4期328-332,共5页
Acta Agrestia Sinica
基金
国家重点基础研究发展规划项目(G2000018601)
