摘要
应用１１种限制性内酶ＢａｍＨⅠ、ＢｓｔＥⅡ、ＥｃｏＲⅠ、ＥｃｏＲⅤ、ＨｉｎｄⅢ、ＫｐｎⅠ、ＰｓｔⅠ、ＳａｌⅠ、ＳｓｔⅠ、ＸｂａⅠａⅠ和ＸⅠⅠ和ＸｈｏⅠ分别将中国棉铃虫核多角体病毒（单粒包埋ＨａＳＮＰＶ）湖北株基因组Ｄ组ＤＮＡ酶切为１０、１２、２２、２１、１３、６、６、４０、６、２１、６个片段，并求得基因组大小平均为１什什Ｍｒ≈７９．１×１０６．以家委核多角体病毒ＢｍＮＰＶ多角体基因为探针，利用Ｓｏｕｔｈｅｒｎ杂交技术将病毒多角体基因定位在ＳａｌⅠ４．２×１０３ｂ左右的片段上．与棉铃虫核多角体病毒其它株系酶切图谱比较结果表明，本株病毒与上海等株系及美洲棉铃虫核多角体病毒ＨａＳＮＰＶＥｌｋａｒ株系酶切图谱相似，它们之间的条带数和大小差异较小，而与已发现的所有多粒包埋型病毒ＨａＳＮＰＶ酶切图主谱差异较大．据此认为ＨａＳＮＰＶ和ＨａＭＮＰＶ属于基因型不同的两类病毒。
Digestion of Helicovrtpa armigera nuclear polyhedrosis virus(HaSNPV Hubei isolate)with 11 restrictionendonucleases,BamH Ⅰ,BstE Ⅱ,EcoR Ⅰ,ECoR Ⅴ,Hind Ⅲ,Kpn Ⅰ,Pst Ⅰ,SalⅠ,SstⅠ,Xba Ⅰ andXho Ⅰ resulted in 10, 12, 22,21, 13, 6, 6, 40, 6, 21 and 6 fragments,respectively.The average Mr of theviral genome was about 79. 1 × 106 (i. e. 119. 8 × 103 b). The polyhedrin gene of HaSNPV was located in a4. 2 × 103 b Sal Ⅰ fragment by using BmNPV Ph sene as a[α-32p]-labelled probe and Southern hybridization method. The restriction Patterns of the viral DNA showed only slight differences in the number and sizeof several fragments with HaSNPV(Shanghai inolate )or HzSNPV(strain Elkar),but greatly varied in thosewith all HaMNPV isolates. This suggested that there was senotypic heterogeneity between HaSNPV andHaMNPV,but high homology among HaSNPV isolates.
出处
《应用与环境生物学报》
CAS
CSCD
1996年第4期387-392,共6页
Chinese Journal of Applied and Environmental Biology
基金
"863"计划资助
关键词
棉铃虫
核多角体病毒
昆虫病毒
限制酶
基因
HaSNPV
restriction endonuclease Pattern
polyhedrin gene localization
homology
