摘要
目的:利用基因芯片技术筛选p27kip1转染乳腺癌MCF-7细胞的差异表达基因,探讨p27kip1调控肿瘤细胞增殖和细胞周期的分子机制。方法:将含p27cDNA的质粒在脂质体介导下体外转染乳腺癌MCF-7细胞,利用Affy-metrix公司的人类基因组基因芯片U133A分别检测表达p27kip1蛋白和空载体的乳腺癌细胞株的基因表达谱,用生物信息学软件进行比较分析,对差异表达基因进行功能分类。结果:在含22277个基因cDNA的芯片上差异表达的基因有1379条,其中表达谱发生明显变化(2倍以上)的基因173条,占总检测基因的0·78%。157条基因表达明显上升(SLR>1),占总检测基因的0·705%;16条基因表达明显下降(SLR<-1),占总检测基因的0·072%。基因表达差异主要集中在物质代谢、细胞增殖、细胞周期调控、细胞分化与凋亡、免疫反应、信号传导、蛋白转运、调节转录等方面。结论:多基因共同参与了p27kip1抑制肿瘤细胞过度增殖以及促进细胞凋亡的作用。对于p27kip1相关基因群的研究有助于认识p27kip1蛋白在细胞周期调控乃至整个细胞癌变过程中作用的分子机制。
OBJECTIVE： To investigate the gene expression profiles related to human breast cancer cell line overexpressing p27^kip1 with cDNA microarray, and to explore the molecular mechanism of p27^kip1 in proliferation and cell cycle of tumor cell. METHODS： The p27^kip1 cDNA was transfected into human breast cancer cell line MCF-7 with lipofectamine. And Affymetrix Human Genome U133A Array was used to screen the differentially expressed genes between overexpression of p27^kip1 gene and the same vector without p27^kip1 gene in human breast cancer cell line. The results were analyzed by bioinformation methods. RESULTS： 1 379 genes were showed to be differentially expressed in cDNA microarray of 22 277 examined genes. Among them, 157 genes were up-regulated and 16 genes down-regulated more than 2-fold. These genes were involved in a number of distinct families, including metabolism, cell proliferation, regulation of cells cycle, cell differentiation, immune response, signal transduction and others. CONCLUSION： These results indicate that numerous genes may be involved in the mechanism that overexpressed p27^kip1 induces apoptosis and suppressive effect on cell growth. The study on p27^kip1 related genes will lead insight into the molecular mechanism of its protein in regulation of cell cycle and oncogenesis.
出处
《中华肿瘤防治杂志》
CAS
2006年第21期1601-1604,共4页
Chinese Journal of Cancer Prevention and Treatment
基金
国家自然科学基金(30470669)
关键词
乳腺肿瘤/病理学
肿瘤细胞
培养的
基因
肿瘤抑制
基因表达
核酸探针
breast neoplasms/pathology
tumor cells,cultured
genes,tumor suppressor
gene expression
nucleic acid probes
