摘要
亚甲基蓝(MB)可捕获Fenton反应产生的羟自由基生成无色加合物,选用亚甲基蓝为槲皮素(Que)及其配合物抗羟自由基活性测定体系的指示剂。实验优化测试条件为:体系pH 8.0,加入H2O2溶液(0.3%)0.50 mL,FeSO4溶液(5 mmol.L-1)0.50 mL和MB溶液(2.56×10-5mol.L-1)1.0 mL。由此建立了测定槲皮素配合物抗.OH活性的光谱测定方法。方法简便,尤其适合于配合物体系抗自由基活性的分析。测定了槲皮素及Que-Zn(Ⅱ),Que-Cu(Ⅱ),Que-Fe(Ⅲ)配合物的抗.OH活性。结果表明3种槲皮素配合物的抗羟自由基活性均比槲皮素高,配合物活性Que-Cu(Ⅱ)>Que-Zn(Ⅱ)>Que-Fe(Ⅲ),表现出金属离子与有机活性配体协同作用可提高其抗氧化活性的能力。
In the present, Fenton-Methylene Blue spectral measurement was established, and the new analytical method was applied successfully to determine the inhibitory effect of quercetin （Que） and its complexes on hydroxyl free radical. Methylene Blue（MB） can capture the · OH produced by Fenton reaction, turning its color from blue to achromatic. Therefore, MB was selected as the indicator of the spectral measure study. The testing conditions optimized were pH 8.0, HzCh solution （0.3%） 0. 50 mL, FeSO4 solution （5 mmol · L^-1） 0. 50 mL and MB solution（2. 56×10^-5 mmol · L^-1） 1.0 mL. The experiment results showed that Que-Zn（ Ⅱ ）, Que-Cu（ Ⅱ ） and Que-Fe（ Ⅲ ） complexes held significant effects of scavenging action on · OH radical and were more effective in scavenging · OH than quercetin itself, showing the good cooperative action between quercetin and trace metal elements. The structure-bioactivity relationship of the coordination complexes was also discussed through analyzing the hntioxygenic activity of quercetin-metal complexes.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2006年第12期2294-2297,共4页
Spectroscopy and Spectral Analysis
基金
国家自然科学基金项目(20375026)
四川省科技厅基金项目(02GY029-038)
重庆市自然科学基金重点项目(CSTC-2004DA7019)资助
关键词
亚甲基蓝光谱法
槲皮素配合物
羟自由基
MB spectral analysis method
Quercetin complex
Hydroxyl free radical
