摘要
目的:探讨紫草素对内皮细胞的作用,明确其抗血管新生机制。方法:利用塞唑兰(MTT)法测定紫草素对内皮细胞增殖的影响,利用迁移实验和体外管腔形成实验观测紫草素对内皮细胞迁移及管腔形成能力的影响。结果:紫草素可在体外抑制内皮细胞的血管新生活性,20μmol·L-1的紫草素对内皮细胞增殖、迁移及管腔形成能力的抑制率分别为38.66%±0.05%、79.69%±0.03%及80.95%±0.05%。结论:紫草素可直接作用于内皮细胞,通过抑制其增殖、迁移及管腔形成而发挥其抗血管新生作用。
AIM： To investigate the effect of shikonin on the endothelial cell and elucidate its anti-angiogenic mechanism. METHODS： MTT assay, cell migration assay and in vitro angiogenesis assay were explored to determine the effect of shikonin on the proliferation, migration and angiogenic ability of endothelial cells respectively. RESULTS： Shikonin can inhibit the angionenesis of endothelial cells in vitro. At 20 μmol· L^-1, the inhibition ratio of the proliferation, migration and angiogenic ability were 38.66% ± 0.05%, 79.69% ± 0.03% and 80.95% ± 0.05% respectively. CONCLUSION： Shikonin can inhibit the angiogenesis through its effect on endothelial cell directly： the suppression of proliferation, migration, angiogenic ability of endothelial cells.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2006年第11期1253-1255,共3页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
上海市卫生局青年基金资助(№044Y11)
关键词
紫草素
内皮细胞
管腔形成
凋亡
shikonin
endothelial cell
tube formation
apoptosis
