摘要
采用RT-PCR技术对我国海南、云南、广西和广东等不同番木瓜生产区的7个番木瓜环斑病毒外壳蛋白基因(PRSV-CP)进行克隆和鉴定分析。PRSV-CP编码区在864～873核苷酸之间,编码288～291氨基酸。对分离的7个PRSV-CP的核苷酸及氨基酸序列的比较结果表明大部分序列差异都处于N端,而各产区番木瓜PRSV-CP基因的3′端586～864bp区段,即278bpDNA片断的同源率最高,达到98.5%。这一同源序列的获得为利用植物基因工程培育广谱抗病性的番木瓜新品系奠定了基础。
The coat protein gene of seven kinds of papaya ringspot virus （PRSV） isolated originally from different plantations in China （ four from Hainan, one from Guangdong, one from Guangxi and one from Yunan） were cloned and sequenced using RT-PCR. The CP-coding region varied in size from 864 to 873 nucleotides, encoding proteins of 288-291 amino acids. The observation of corresponding heterogeneity in the CP length showed that all the differences in length were confined to the N terminus of the CP-coding region. The higher sequence identity regions was found in 586- 864 nt of the seven PRSV CP-coding regions of C terminus. The information of CP gene will be useful in designing and developing durable virus resistant transgenic papaya in China.
出处
《华南热带农业大学学报》
2006年第4期1-5,共5页
Journal of South China University of Tropical Agriculture
基金
国家自然科学基金(30560082)
国家转基因植物研究与产业化开发专项(JY04-B-02)
关键词
番木瓜环斑病毒
外壳蛋白基因
克隆
序列比较
Papaya ringspot virus
coat protein gene
cloning and sequence analysis
