摘要
选取分别被克隆到Ｃｏ２４质粒和ＢＳ质粒上的番木瓜环斑病毒外壳蛋白（ＰＲＳＶ—ＣＰ）基因和柞蚕抗菌肚Ｄ（ＣｅｃｒｏｐｉｎＤ）基因为材料，构建了单抗表达载体ＦＫ３和ＰＣｏ２５，进一步构建了双抗植物表达载体ｐＫＰＴ。这为开展单抗和多抗植物基因工程的研究提供了重要途径。
Papaya ringspot virus coat protein(PRSV CP)gene and.Antheraea,pernyi Cecropin D gene were cloned into plasmids Co24 and BS respectively-and then used as materials for the construction of resistant expression vect-ors pK3 and pCo25 and, further,of,digenous resistant expression vector pKPT by gene conjunction. This is an important method to study plantresistant gene engineering.
出处
《热带作物学报》
CSCD
1994年第S1期49-54,共6页
Chinese Journal of Tropical Crops
关键词
番木瓜
环斑病毒外壳蛋白
柞蚕抗菌肽D
双抗表达载体
Papaya Ringspot virus coat protein Antheraea pernyi cecropin D Digenous resistant expression vector.
