摘要
目的观察糖基化终产物(AGEs)在肾脏组织中的沉积及其与肾脏固有细胞表型转化的关系。方法采用免疫组化染色法观察AGEs:羧甲基赖氨酸(CML)、吡咯素、戊糖苷素和细胞骨架蛋白:α-肌动蛋白(-αSMA)、L-收缩平滑肌蛋白(L-CLD)在28例2型糖尿病肾病(DN)患者肾活检组织(疾病组)的沉积,分析二者间及其与肾脏病理改变和临床表现的关系。4例增生硬化型IgA肾病及4例轻微病变型和2例正常肾组织作为对照组。结果(1)四组均可见CML、吡咯素分别沉积在系膜区和肾间质,但对照组明显弱于DN组;戊糖苷素只沉积在DN肾小球基底膜(GBM)。(2)系膜区-αSMA、L-CLD的表达与戊糖苷素呈正相关,吡咯素的沉积与间质L-CLD有关。(3)-αSMA和L-CLD及戊糖苷素的沉积与肾小球硬化指数、间质纤维化百分数及蛋白尿、Scr正相关。结论戊糖苷素在GBM的沉积是糖尿病肾小球硬化的重要原因;AGEs对肾脏的影响部分可能是通过引起肾脏固有细胞的表型转化而起作用的。
Objective To investigate the distribution of AGEs in renal tissue and to study the interrelationship of AGEs and phenotypie changes of kidney cells in patients with diabetic nephropathy （DN）. Methods The locations of AGEs and eytoskeletal proteins were evaluated by immunohistochemical method and the relationship between them were analyzed in 28 DN patients. Results The carboxymethyl lysine （CML） and pyrraline were localized in mesangial area （especially in nodular are a） and renal interstitium respectively and the similar areas of positive staining were found in IgA nephropathy, minor lesion and normal renal tissue, but in less degree of staining. Pentosidine was localized only in glomerular basement membrane（GBM） in DN and this deposition significantly correlated with diabetic glomeruloselerotie index, 24-h urinary protein and serum ereatinine level. The positive staining of s-smooth muscle aetin （a-SMA）, and low molecular caldesmon （L-CLD）were found in the mesangial area, and the expression significantly correlated with pentosidine, but not with CML. The SMA in interstitial area significantly correlated with pyrraline. Conclusions AGEs are involved in renal injury in DN. Their effeet on kidney is, at least in part, produced via phenotypie changes of intrinsic cells. Pentosidine deposition in DN may be specific.
出处
《中国糖尿病杂志》
CAS
CSCD
北大核心
2006年第6期438-441,共4页
Chinese Journal of Diabetes
关键词
糖尿病肾病
糖基化终产物
表型转化
Diabetic nephropathy
Glycosylation end products, advanced
Phenotype transformation
