摘要
用乙醇对香椿叶粉末进行提取,树脂柱浓缩,真空冷冻干燥,甲醇溶解,制备香椿黄酮。用7.6 mol.L-1盐酸85℃水浴回流2 h进行水解,以反相ODS柱甲醇-水(体积比为50:50,磷酸调pH至2.53)为流动相,在波长368 nm处对香椿叶中的槲皮素进行分离、测定。结果表明:香椿叶中含槲皮素平均质量分数为1.28%,平均加样回收率为98.9%,RSD为1.75%,反相高效液相色谱法测定香椿叶中槲皮素含量操作简便易行、结果准确可靠。
A RP - HPLC method for the determination of quercetin in leaves of Toona sinensis was developed. Samples were mixed with HCl of 25% , which were heated at 85℃ for 2 hours. Quercetin was separated completely on an ODS column（4.6mm ×250mm）, using a mixture of methanol and water （50： 50, phosphoric acid made the pH 2.53） as mobile phase, with UV detection at 368nm. The results have shown that the content of quercetin was up to 1.28% ,the recovery rate was 98.9% and RSD was 1.75%. The method is quick and simple with good accuracy and can be used for the quality control.
出处
《氨基酸和生物资源》
CAS
2006年第4期74-76,共3页
Amino Acids & Biotic Resources
关键词
香椿叶
槲皮素
反相高效液相色谱
leaves of Toona sinensis
quercetin
reversed - phase high performance liquid chromatography
