摘要
目的测定不同产地蜂胶中柯因、槲皮素、洋芹素的含量并比较其差异。方法利用高效液相色谱(HPLC)法以柯因、槲皮素、洋芹素为指标成分测定河南、湖北等地6种蜂胶中相应成分的含量。色谱柱为Hypersil-ODS(150 mm×6mm,5μm),流动相为甲醇∶水∶磷酸(168.75∶44.8∶0.2),流速为0.7 m l/m in,检测波长为273 nm,柱温为26.8℃,用外标峰面积法定量。结果不同产地蜂胶中指标成分的含量不同。结论该法简便、准确、可靠、重现性好,可用作蜂胶中柯因、槲皮素、洋芹素的含量检测和不同产地蜂胶成分的对比以及真伪优劣的鉴定。
Objective To determine the contents of Lomefloxaein Hydrochloridum, Quercetin and Apigenin in propolis from different production areas and compare their discrepancies. Methods The contents of Lomefloxacin Hydrochloridum, Quercetin and Apigenin in 6 kinds of Propolis from Henan and Hunan were determined by HPLC with Hypersil-ODS column. The mobile phase was the mixture of methanol-water-phosphate（ 168.75：44.8：0.2） with the flow rate of 0.7 mL/min. The detection wavelength was 273 nm and the column temperature 26.8℃. External standard method was used for measurement. Results The propolis from different production areas has different quota composition. Conclusion This method is simple, accurate and with good reproducibility, which can be used to determine the contents of Lomefloxacin Hydrochloridum Quercetin and Apigenin in Propolis, compare with the composition of propolis from different areas, distinguish their genuine and sham, good and bad.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2006年第12期2501-2502,共2页
Lishizhen Medicine and Materia Medica Research
关键词
蜂胶
高效液相色谱
柯因
槲皮素
洋芹素
Propolis
HPLC
Lomefloxacin Hydrochloridum
Quercetin
Apigenin
