摘要
[目的]探讨骨质疏松症骨胶原的变化及其与骨生物力学相关性。[方法]7个月龄未交配雌性SD大鼠30只,随机平分为:OVX组(卵巢切除组)和Sham组(假手术组,未切除卵巢)。术后12周处死大鼠,行第L5压缩力学实验,并测定L5羟脯氨酸(HYP)含量,同时应用m asson三色法行L5切片染色,光镜下观察胶原纤维形态学的改变。[结果]卵巢切除12周后,Sham组HYP含量(0.197±0.035)mg/g,镜下见胶原纤维排列规则,走向清晰连续性好,胶原纤维粗壮,呈致密板层样。OVX组大鼠L5HYP含量为(0.182±0.022)mg/g,镜下见胶原纤维排列稀疏紊乱,变细甚至断裂溶解,连续性差。两组HYP含量相比P<0.05。OVX组HYP变化与L5弹性模量、抗压强度及最大压缩力变化有直线相关性。[结论]卵巢切除致骨质疏松,骨胶原含量减少和质量下降。骨胶原含量下降与骨生物力学强度降低有直线相关性。骨质疏松骨折与骨胶原含量下降有关。
[ Objective] To investigate the changes and correlation of bone collagen and biomechanical property in osteoporosis rats. [ Method ] Normal 30 nullimating female 7 month SD rats were divided randomly to two groups, each included 15. The sham group was unovariectomized, and the OVX group was ovariectomized. To execute the rats after 12 weeks, the author get the 5th lumbar vertebra, and then detected the biomechanical property and HYP. The author observed the collagen morphology of the 4th lumbar vertebra using the microscope after the masson triad color dyeing. [ Result ] Twelve weeks later, HYP in the sham group was （0. 197 ±0. 035） mg/g. The bone collagen was regular, compact, and closed succession. HYP in the OVX group was （0. 182 ±0. 022） mg/g. The bone collagen was incompact, thin, collapse and poor succession. There was significant change of the HYP in two groups （P 〈0. 05 ） . There was linear correlation between the HYP and the max condensing force, Young＇s modulus and crushing strength in the OVX group. [ Conclusion] The ovariectomized rats can be developed into osteoporosis, and the bone collagen in the ovariectomized rats is decreased. There is linear correlation between the bone collagen and the biomechanical intensity descending. There is correlation between the osteoporosis fracture and the bone collagen.
出处
《中国矫形外科杂志》
CAS
CSCD
北大核心
2006年第23期1807-1809,共3页
Orthopedic Journal of China
基金
山东省科技厅重点项目(No.2004GG2202163)
