摘要
21个小白菜品种小孢子培养结果表明：适宜的花蕾长为2．5—3．5mm，花瓣与花药长度比为1／2，3／4；0．05～0．10mg·L^-1的细胞分裂素能促进小孢子胚的发生，高于此浓度的细胞分裂素对小孢子胚的产生有抑制作用，并使小孢子胚向畸形化发展；0．05mg·L^-16-BA＋0．10mg·L^-1NAA的配比可以明显提高成熟胚的比例。
Twenty-one varieties of Brassica campestris, ssp. chinensis L. were used for isolated microspore culture. It was found that the bud size with 2.5- 3.5mm and petal/anther with 1/2-3/4 could grow into division. 0.05-0.1 mg·L^-1 BA could promote the embryogenesis and improve the frequency of microspore embryogenesis. Higher contents of ctytokinin had inhibition to embryoid induction and made deformity embryos. The media added 0. 05mg·L^-16-BA and 0. 10 mg·L^-1NAA could enhance the ratio of mature embryos evidently.
出处
《沈阳农业大学学报》
CAS
CSCD
北大核心
2006年第5期763-765,共3页
Journal of Shenyang Agricultural University
基金
国家"863"项目(2004AA241120)
关键词
小白菜
小抱子培养
胚状体
诱导率
Brassica chinensis
isolated microspore culture
embryoid
frequency of microspore-derived embryos
