摘要
目的:研究运动训练对脑梗死大鼠行为学的影响并从神经可塑性角度探讨其机制。方法:用线栓法制作大鼠大脑中动脉闭塞(MCAO)脑梗死模型,56只Wistar大鼠随机分为假手术组、模型组和康复组。康复组于手术后5天开始进行滚筒、转棒、平衡木、网屏训练,每日40min,每周6次,共5周,假手术组与模型组则维持原来生活状态。对大鼠进行神经功能、运动能力和学习记忆能力测评观察其行为学变化,免疫组化法观察缺血区神经丝蛋白200(NF200)的表达变化。结果:康复组大鼠神经功能、运动能力、学习记忆能力优于模型组,神经功能在3周差异有显著性意义(P<0.05),其转棒实验和平衡木实验在3周、5周(P<0.01—0.05)差异有显著性意义,网屏实验在3周、5周差异有显著性意义(P<0.05),学习记忆能力在5周有极显著性意义(P<0.01)。康复组大鼠缺血区NF200蛋白质阳性表达较模型组增多,在3周时差异有显著性意义(P<0.05),5周时有极显著性意义(P<0.01)。结论:运动训练促进脑梗死大鼠神经功能、运动能力、学习记忆能力的恢复,其机制可能与缺血区NF200蛋白质表达上调有关。
Objective：To study the changes of behavior and expression of neurofilament-200 （NF200） in cerebral infarction rats after motor training. To discuss the mechanism of behavior rehabilitation and the plasticity of the central nervous systems （CNS）. Method：An animal model was made by the middle cerebral artery occlusion（MCAO）. Fifty-six 2-month old male Wistar rats were divided into 3 groups randomly： 8 rats in sham-operated group （sham）, and 24 rats in rehabilitation group and model group respectively. Motor training（balancing, grasping, rotating and walking exercises） were applied in rehabilitation group from post operation 5d, 40min/d, 6 times per week, for 5weeks while those in sham group and model group were kept in their original living state. Bederson neural function, balancing wood, rotating bar, and net screen were tested at the 1st, 3rd and 5th week after operation respec- tively. The expression of NF200 in ischemic area was observed immunohistochemlcally. Result：Bederson neural function in rehabilitation group exceeded that in model group, showing significant differences at the 3rd week （P〈 0.05）. Motor ability in rehabilitation group was better than that in model group. Compared with model group, the function of rotating bar and balancing wood in rehabilitation group was significantly different at the 3rd week （P〈 0.01） and the 5th week （P〈O.05）; net screen function was significantly different at the 3rd and 5th week （P〈0.05）. Both learning and memory abilities were improved obviously in rats after motor training （P〈0.01）. Conclusion：Motor training can promote the recovery of neural function, ability of motor, learning and memory in cerebral infarction rats, which may be related to NF200 expression upregulation in brain ischemic area.
出处
《中国康复医学杂志》
CAS
CSCD
北大核心
2006年第11期980-984,I0001,共6页
Chinese Journal of Rehabilitation Medicine
