摘要
以放射性配基结合分析法对正常成年小鼠大脑皮质中Ｎ─甲基－Ｄ－天冬氨酸（ＮＭＤＡ）受体作了鉴定；观察了衰老小鼠ＮＭＤＡ受体、空间辨别能力、海马突触传递长时程增强（ｌｏｎｇｔｅｒｍｐｏｔｅｎｔｉａｔｉｏｎ，ＬＴＰ）的变化及补肾中药复方对这些变化的影响。结果表明：小鼠大脑皮质含有丰富的、高亲和力的ＮＭＤＡ受体；衰老过程中小鼠ＮＭＤＡ受体的最大结合容量（Ｂｍａｘ）呈渐进性降低，空间辨别能力下降，ＬＴＰ的振幅和斜率明显降低；补肾中药复方具有提高衰老小鼠ＮＭＤＡ全体Ｂｍａｘ值和维持ＬＴＰ于较高水平的作用。
By means of radioligand binding assay,the
N-methyl-D-aspartate(NMDA) receptor wasidentified in the cerebral cortex of mature mice, and
the change of NMDA receptors in aged micewas observed.Meanwhile the influence of
kidney-tonifying apozem consisting of eight kinds ofChinese medicinal herbs on this change
was investigated. The results demonstrated that abundantNMDA receptors with high affinity
existed in the cerebral cortex of mice. In aged mice themaximal binding capacity(Bmax)of
NMDA recptors and the amplitude and slope of long termpotentiation(LTP)decreased markedly.
Administration of kidney-tonifying apozem could im-prove the changes of NMDA recptors and
LTP in aged mice. Upon analyzing the results ob-tained,the following conclusions can be
reached:(1) The change of NMDA receptors may be re-lated with the decline of learning and
memory ability during aging.(2) Administration ofkidney-tonifying apozem can improve the
ability of leaming and memory during aging.
出处
《中国应用生理学杂志》
CAS
CSCD
1994年第1期49-53,共5页
Chinese Journal of Applied Physiology
关键词
大脑皮质
衰老
天冬氨酸
受体
radioligand binding assay
NMDA
receptor
mice cerebral cortex
aged
LTP.
