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水稻类受体激酶OsCR4的抗体制备及特异性检测 被引量:5

Preparation and Identification of Antibody Against OsCR4,the Receptor-like Kinase from Rice
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摘要 运用生物信息学的方法对水稻类受体激酶OsCR4的抗原性进行分析,选取胞外部分片段与GST融合,在大肠杆菌中大规模诱导该融合蛋白,利用GST-Sepharose进行亲和层析纯化,所得蛋白组分经SDS-PAGE,结合KCl/DTT染色切胶的方法得到收集.以此融合蛋白作为抗原免疫新西兰兔,获得了1∶512000效价的多克隆抗体,该抗体可特异识别水稻叶片微粒体组分中的OsCR4蛋白.
出处 《中国生物化学与分子生物学报》 CAS CSCD 北大核心 2006年第11期924-930,共7页 Chinese Journal of Biochemistry and Molecular Biology
基金 国家自然科学基金资助项目(No.30570153) 国家高技术研究发展计划(863计划 No.2002AA2Z1001-10)~~
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