摘要
本试验采用RT-PCR的方法对戊型肝炎病毒大庆株(DQ1HEV株)的全基因进行分片段扩增,并对其两个末端采用末端快速扩增法(RACE)进行扩增、克隆,测序。与已报道的人的14株HEV的4个基因型的核苷酸和氨基酸进行比较,与IV型HEV的同源性最高。ORF1区与IV型HEV核苷酸的同源性为82.6%～83.6%,氨基酸同源性为93.5%。ORF2区与IV型HEV核苷酸的同源性为87.0%～88.4%,氨基酸同源性为95.8%～97.4%。ORF3区与IV型HEV核苷酸的同源性为94.4%～96.5%,氨基酸同源性为90.3%～96.5%。其结果表明DQ1HEV株为IV型HEV。
One HEV isolate from a swine in Daqing were sequenced over the entire genome by RT-nested PCR and the 5＇ and 3＇ends were amplified with RACE, and compared phylogenetically along with 14 reported HEV isolates, The data showed that HEV in swine in Daqing was genetically closely related to human HEV genotype IV.The full length sequence of HEV DQI showed identities of 82.6 % to 83.6 %, 87.0 % to 88.4 %, 94.4 % to 96.5 % with HEV genotype IV in ORF1, ORF2, ORF3, respectively. ORF1 protein of HEV DQ1 was 93.5 % identical to that of HEV genotype IV. ORF2 and ORF3 protein of HEV DQ1 were 95.8 % to 97.4% and 90.3 % to 96.5 % identical respectively to that of HEV genotype IV. These findings suggest that swine HEV DQ1 comes from HEV genotype IV.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2006年第5期507-510,共4页
Chinese Journal of Preventive Veterinary Medicine
关键词
猪戊型肝炎病毒
克隆
序列分析
swine hepatitis E virus
cloning
sequence analysis
