摘要
目的:探讨己酮可可碱(PTX)对脂多糖(LPS)诱导的内皮细胞单层通透性的保护作用。方法:建立内皮细胞单层损伤模型,随机分为LPS组、PTX组、LPS+PTX组、对照组,测定内皮单层滤过系数、蛋白质渗透压反射系数及进行内皮细胞单层形态学检查。结果:LPS组内皮单层滤过系数显著升高、蛋白质渗透压反射系数显著降低;与正常对照组比较,PTX组的滤过系数和蛋白质渗透压反射系数无明显变化;与LPS组比较,LPS+PTX组两系数及内皮细胞单层形态学检查无明显变化,近似于正常对照组。结论:PTX无降低正常内皮细胞单层通透性的作用;PTX对LPS诱导的内皮细胞单层通透性有保护作用,其机制可能与PTX拮抗LPS导致内皮细胞单层通透性增加的某些环节有关。
Objective：To study the effects of pentoxifylline （PTX） on LPS-induced endothel cell monolayer permeability. Methods：To observe the influence of PTX on the permeability of in vitro cultured endothelial cell monolayer （ECM） in LPS-induced ECM injury model with human umbilical vein endothelial cells（HUVEC） in LPS group, PTX group , LPS＋ PTX group and control group by determining the changes of the filtration coefficients （Kf） and the osmotic reflection coefficients （σ） , and the histological appearance of endothelial cell monolayer under microscope. Resuit：PTX in LPS＋ PTX group could markedly decrease the Kf of ECM , enhance the osmotic reflection coefficients of that compared with those in LPS group. Conclusion.PTX could protect LPS-induced endothel cell monolayer permeability.
出处
《广西医科大学学报》
CAS
北大核心
2006年第5期712-713,共2页
Journal of Guangxi Medical University
基金
广西自然科学基金资助项目(桂科自No.0135057)
关键词
己酮可可碱
内皮细胞单层通透性
脂多糖
pentoxifylline
endothelial cell monolayer permeability
lipopolysaccharide
