摘要
建立了利用原子光谱技术测定人肺组织中55种元素的方法。样品采用冷消化过夜，温控电热板消解。其中49种元素利用电感耦合等离子体质谱（ICP-MS）进行测定；As和Se由原子荧光分光光度计（AFS）测定，K，Na，Ca和Mg由原子吸收分光光度计（AAS）测定。24个元素的测定值与牛肝和人发两种标准物质中的参考值基本吻合。除了Cr，Er和U三元素回收率略为偏高外，其余30种元素的加标回收率均在90％～110％之间。绝大多数元素的精密度在1．7％～10%之间。统计学分析结果表明，共有20种元素的含量在所测17对癌组织和癌旁组织之间具有显著性差异。此方法简便、快速、准确。
A method for determining 55 elements in human lung tissue was developed. Mixed acid （HNO3： HClO4 ） was added into samples, which were digested at room temperature over night, then heated at 180 ℃. Arsenic and selenium in lung tissue were determined by hydride generation atomic fluorescence spectrometry （HC-AFS）, potassium, sodium, calcium and magnesiurn were determined by atomic absorption spectrometer （AAS）, while the rest of forty-eight elements were determined by inductively coupled plasma mass spectrometry （ICP-MS）, respectively. Reference materials of GBW（E）080193 bovine hepar and GBW09101 human hair were analyzed by the described method. The measured element values in two reference materials accorded with their reference values. The recovery rates for most of the studied elements were 90%-110%. The preeisions of the method were 1. 7%-10. 0%. The concentrations of seventeen elements in the carcinomatous tissues were remarkably different from those in the pericareinomatous tissues. The method is rapid, simple and accurate.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2006年第11期2120-2124,共5页
Spectroscopy and Spectral Analysis
基金
教育部留学回国启动基金(2003)资助项目
关键词
肺癌
原子光谱分析
多元素
Lung cancers
Atomic spectrometry
Elements
