摘要
目的:研究终末期肾病(ESRD)患者Th1/Th2型细胞因子的特征及其与外周血T细胞凋亡的关系,并探讨不同透析膜对T细胞凋亡的影响。方法:应用流式细胞术检测10例ESRD未透析(ND)患者,维持性血液透析的患者45例,分别采用醋酸纤维素膜(CA)、低通聚砜膜(PS-LF)、高通聚砜膜(PS-HF)进行透析,以及8例健康对照组(HC)的外周血T细胞,经PHA刺激培养24h后锚定蛋白(AnnexinV)的表达。用ELISA法检测细胞培养上清IFN-γ及IL-4的水平。结果:ESRD患者未透析组及3种膜透析组T细胞AnnexinV的表达高于健康对照组(P<0.05)。各透析组患者T细胞AnnexinV的表达以CA组最高,PS-HF组最低,组间比较具有统计学意义(P<0.05);ESRD患者各组IFN-γ的水平均低于健康对照组(P<0.05),与AnnexinV的表达呈负相关;IL-4的水平高于健康对照组(P<0.05),与AnnexinV的表达呈正相关。结论:ESRD患者存在Th型细胞因子的失衡,呈Th2型细胞因子占优势,T细胞的凋亡增加。
AIM： To investigate the characterization of Th1/Th2 profile in patients with end-stage renal disease （ESRD） and the correlation with the apoptosis of the peripheral blood T cells; and to sduty the influence of different dialysis membranes on the apoptosis of T lymphocyte. METHODS： T cells from 10 non-dialyzed （ND） patients, 45 maintenance hemodialysis patients with cellulose acetate （CA） membrane, low-flux （PS-LF） and high-flux polusulfone （PS-HF） membrane, and 8 healthy volunteers （HC） were seperated and stimulated with PHA for 24 hours in vitro. Then the apoptosis of T cells and supernatants levels of IFN-γ and IL-4 were detected by Flow cytometry （FCM） and ELISA. RESULTS： In ESRD patients, the expression of Annexn V in T lymphocyte was higher than that of group HC （P 〈 0.05）, group CA was higher than group PS-HF and PS-LF （ P〈0.05）. The level of IFN-γ, of ESRD patients was decreased significant compared with that in group HC （P 〈 0.05 ）, and there was negative correlation, between the Annexn V and IFN-γ. IL-4 was increased in ESRD patients （P 〈0.05） and it was positive correlated with Annexin V. CONCLUSION： ESRD patients showed suppressed secretion of IFN-γ, increased secretion of IL-4 and apoptosis of T lymphocytes.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2006年第6期763-766,共4页
Chinese Journal of Cellular and Molecular Immunology
