摘要
目的:探讨体外分离培养的小鼠骨髓来源的成熟与未成熟DC中核转录因子RelB(avianreticuloendotheliosisviral(v-rel)oncogenerelatedB)基因的表达。方法:无菌从C57BL/6小鼠股骨和胫骨中取出骨髓细胞,利用rmGM-CSF和rmIL-4联合诱导骨髓前体细胞产生未成熟的DC,未成熟的DC在培养结束前18h经LPS刺激获得成熟的DC,用流式细胞术分析它们的表型,用RT-PCR和免疫荧光染色法检测成熟与未成熟DC中,RelBmRNA和其蛋白的表达。结果:流式细胞术分析显示未成熟的DC中MHC-Ⅱ类分子和共刺激分子(CD86和CD40)呈低水平表达;而成熟的DC则呈高水平表达。RT-PCR和免疫荧光染色法检测结果均显示,RelB基因在未成熟的DC中呈低水平表达;而在成熟的DC中呈高水平表达,两者比较具有统计学意义(P<0.01)。结论:RelB基因的表达与小鼠骨髓来源的DC的成熟状态密切相关。抑制DC中RelB基因的表达,有可能诱导产生具有耐受原性的未成熟的DC。
AIM： To explore the expression of avian reticuloendotheliosis viral （v-rel） oncogene-related B （RelB） mRNA in vitro in murine mature and immature myeloid dendritic cells （DCs）, METHODS： The bone marrow was collected from the femur and tibias of C57BL/6 mice in sterile condition, Bone marrow precursors were cultured with recombinant mouse granulocyte-macrophage colony-stimulating factor （rmGM-CSF） and interleukin-4（rmIL-4） to produce immature DCs. Immature DCs were stimulated by LPS 18 h before the end of culture to become mature. DCs＇ phenotype was detected by flow cytometry （FCM）, RelB expression and protein level were determined by RT-PCR and immunofluorescence staining, RESULTS： The expression level of co-stimulatory molecules （ CD86 and CD40 ） and MHC-II class molecule were low in immature DCs, whereas high in mature DCs, RelB expression was significantly higher in mature DCs than in immature DCs （P〈0.01）. CONCLUSION： RelB expression is closely associated with the maturative situation of DCs. Inhibition of RelB expression in DCs may induce tolerogenic DCs.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2006年第6期703-705,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
广东省自然科学基金资助项目(04020404)
关键词
RelB基因
小鼠
骨髓树突状细胞
表达
RelB gene
mouse
bone marrow-deriveddendritic cell
expression
