摘要
运用气相色谱法、液相色谱法、分光光度法和碘量法检测面粉中过氧化苯甲酰(BPO)的含量,并比较这4种方法的回收率、精密度和综合效能。结果表明:气相色谱法的回收率为96.35%,相对标准偏差(RSD)为3.2%,能够满足日常检测分析要求,但试验操作复杂,在洗涤和提取的过程中需要试验人员的熟练操作和丰富经验;高效液相色谱法的回收率最高,为98.67%,但是成本较高,仪器设备比较昂贵;碘量法的滴定终点较难判断,故准确度较低,但可以快速估测;分光光度法方法简单,成本较低,且准确度和回收率都能够达到要求,适合一般企业和检测部门应用,但对陈面粉的检测效果较差。
Four methods used in the determination of the amount of benzoyl peroxide in wheat flour, such as gas phase chromatography（GC）, highly pressure liquid phase chromatography（HPLC）, spectrophotometry and iodimetry, were compared in returns-ratio, precision and comprehensive efficiency. It showed that the returns-ratio and RSD of GC method were 96.35% and 3.2% respectively, which was satisfied the daily examination, but the process was complex and needed skilled operation and the rich experience in the experimental process of wash and distilling. The returns-ratio of HPLC was higher, reaching 99.01%, but the cost was also higher and the instrument was very expensive. The iodimetric titration was difficult in judging the end point so the precision was lower, only appreciated for the fast estimation. The spectrophotometer process was simple, the cost was low, and also, the precision and the returns-ratio could meet the examination＇ s requirements, which was suitable for the common application, but was not good for the detennination of the dated wheat flour.
出处
《安徽农业科学》
CAS
北大核心
2006年第21期5465-5466,5517,共3页
Journal of Anhui Agricultural Sciences
关键词
过氧化苯甲酰
增白剂
回收率
精密度
Benzoyl peroxide
Brightening agent
Returns-ratio
Precision
