摘要
目的:从形态学角度观察探讨自由基清除剂对PrP106-126诱导的分化PC12细胞损伤的保护作用。方法:应用光镜、激光共聚焦显微镜技术和MTT法,体外观察不同浓度自由基清除剂对PrP106-126诱导的分化PC12细胞形态学及细胞存活率改变的影响。结果:不同浓度的依达拉奉(15、30、45、60μmol·L-1)对诱导分化的PC12细胞均有保护作用,其保护作用呈剂量依赖关系。激光共聚焦显微镜双荧光标记结果显示,随着依达拉奉浓度的增高,PrP106-126诱导的分化PC12细胞坏死及凋亡的程度降低。结论:依达拉奉对PrP106-126诱导的分化PC12细胞具有抗氧化损伤保护作用。
Aim： To explore the protection of free radical scavenger （edaravone） for morphology of the differentiated PC12 cells infected by prion protein 106-126 peptide. Methods： The protection of effect of edaravone on the differentiated PC 12 cells infected by prion protein 106-126 peptide in vitro. All these changes in cells with 15, 30, 45, 60 μmol·L^-1 in edaravone were dynamically observed. They can be seen under inverted phasecontrast microscope and a laser scanning confocal microscope （LSCM）. They also can be seen by MqT assay. Results： The inverted phasecontrast microscope and LSCM results showed edaravone could significantly protected the differentiated PC 12 cells infected by prion protein 106-126 peptide when these cells were exposed to edaravone at 15, 30, 45, 60 μmol·L for 24h, and it was in a dose dependent manner. Conclusion： Edaravone may prevent the differentiated PC 12 cells infected by prion protein 106-126 peptide from injured of oxidative stress.
出处
《中国临床神经科学》
2006年第6期610-615,共6页
Chinese Journal of Clinical Neurosciences
