摘要
目的:了解幽门螺杆菌(H pylori)感染在农村居民的感染率和探索H pylori感染的传播途径．方法:2004-09对河北省遵化市某村71户家庭263人进行横断面研究,采用面对面问卷调查形式由专人填写调查表,采集静脉血液标本5 mL,用酶联免疫吸附实验(ELISA)方法检测HpUIgG抗体．用免疫印迹方法(Western blot)检测血清中的H pylori抗体．结果:该地区居民的H pylori感染率属于中等水平,男女感染率没有差别．当地居民H pylori感染的危险因素有未成年时住房拥挤、条件差、养猪、现在家庭中几代共同进餐及醋和水果进食过少等．263位受检者中,H pylori总血清感染率为57．41％,抗体平均水平为0．911±0．810μg/L;其中男性感染率为60．50％,抗体平均水平为0．948±0．843μg／L;女性感染率为54．86％,抗体平均水平为0．880±0．784μg／L．H pylori感染有家庭聚集性．父母双方或者一方H pylori感染阳性的其子女H pylori感染率(60％,51．35％)明显高于父母H pylori感染均阴性者(11．11％,P<0．05)．以家庭为单位进行的血清Western blot分析发现各种血缘关系家庭成员,其血清抗体平均相似度较之家庭间成员的平均相似度并没有差别(F=1．22,P>0．05),两两比较结果亦无差别．结论:该地区居民的H pylori感染率属于中等水平,男女感染率没有差别．H pylori感染存在家庭聚集性．
AIM： To estimate the prevalence of H pylori infection in rural families, and to investigate the transmission route of H pylori infection.
METHODS： A cross-sectional study was carried out among 263 villagers from 71 families in September 2004 in Zunhua city, Hebei province. Each subject was interviewed using a unified questionnaire. Blood sample of 5 mL was collected for the antibody examination by enzymelinked immunosorbent assay （ELISA）. Western blot analysis was performed to distinguish the infected strains, and compared with two standard strains NCTC 11637 and CAPM N111, respectively. Similarity analysis was then carried out with Quantity one 4.1.1 software, which is special for photographs of electrophoresis.
RESULTS： The seroprevalence of H pylori infection in residents of this area was of medium level in our country, and it was not associated with gender. In this area, the risk factors of infection were bad living conditions, overcrowding and possession of pigs during childhood, as well as several generations eating together, less vinegar and fruit intake. The overall prevalence and serum level of anti-H pylori antibodies in 263 subjects was 57.41% and 0.911 ± 0.810 μg/L, respectively. In males, they were 60.50% and 0.948 ± 0.843 μg/L, while 54.86% and 0.880 ± 0.748 μg/L in females, respectively （P 〉 0.05）. Infection clustered within families, and 60% and 51.35% of the children with two or at least one infected parent were H pylori-positive, while the prevalence rate in the children with both parents uninfected was only 11.11% （P 〈 0.05）. Western blot showed that the antibodies of the residents living in the area were similar in bands （F = 1.22, P 〉 0.05）.
CONCLUSION： The prevalence of H pylori in residents of this area was of medium level in our country. Infection clusters within families, and the status of parents＇ infection can affect that of the children.
出处
《世界华人消化杂志》
CAS
北大核心
2006年第30期2948-2953,共6页
World Chinese Journal of Digestology
