摘要
目的建立蓬子菜药材的HPLC指纹图谱。方法以香叶木苷为内标物,采用高效液相色谱法分析不同产地10个蓬子菜样品,并进行相似度计算。色谱条件Hypersil ODS2柱(200mm×4.6mm,5μm);流动相为甲醇-0.4%醋酸溶液梯度洗脱;体积流量为1.0mL/min;检测波长340nm;柱温26℃。结果本研究建立了具有19个共有峰的蓬子菜药材指纹图谱,各色谱峰分离较好,达到指纹图谱要求。结论本方法重现性好,可用于不同产地蓬子菜药材的指纹图谱的测定和质量评价。
Objective To establish the HPLC-FPC of Herba Galii Veri. Methods To analyze ten samples from various habitats by HPLC method. The similarity degree was calculated, taking diosmin as the internal standard. Conditions： Column, Hypersil ODS2 （200 mm×4. 6 mm, 5 μm）; mobile phase. mixture liquid of CH3OH-0. 4% CH3COOH in a gradient mode; flowing rate, 1.0 mL/min; wavelength： 340 nm; column temperature, 26 ℃. Results The HPLC-FPC of Herba Galii Veri. was set up by establishing 19 common peaks. The peaks in the spectrum were all separated perfectly, which met the regulation of HPLC-FPC. Conclusion The method has good reproducibility, and can be used in setting up HPLC-FPC and quality control of Herba Galii Veri. from various habitats.
出处
《中草药》
CAS
CSCD
北大核心
2006年第11期1723-1726,共4页
Chinese Traditional and Herbal Drugs
基金
黑龙江省科技攻关计划项目(20031101)
