摘要
目的研究杜仲3种活性部位对小鼠睡眠改善与免疫调节的作用及相互关系。方法通过观察记录日间小鼠睡眠行为状态,戊巴比妥钠阈下催眠剂量实验,延长戊巴比妥钠睡眠时间实验,酶联免疫吸附反应(ELISA)分别检测服用3种不同极性的提取部位、阳性对照物和空白对照组的小鼠脑组织中IL-1β含量,并结合脏器指数等综合分析。结果服用杜仲提取部位的3组小鼠与阳性对照组相比,对睡眠行为状态的影响表现在服药后起效的时相不同;提高戊巴比妥钠阈下催眠剂量睡眠发生率,缩短戊巴比妥钠诱导睡眠潜伏时间并延长睡眠时间;脑内IL-1β含量降低。结论杜仲3种不同活性提取部位具有改善小鼠睡眠、调节免疫的作用,其中乙酸乙酯活性部位具有更好的调节功能。
Objective To study the hypnotic and immunoregulation effects of different active ingredients of Eueommia ulmoides Oliv. bark on mice,and to investigate the interaction. Methods After taking triazolam and distilled water respectively,the mice sleeping-behavior state was observed and recorded;the ratio of sleeping mice was recorded after taking different of ingredients Eucommia ulmoides Oily. combining with pentobarbital sodium at subliminal dose,and mice latent period and sleeping time at supraliminal dose. Measure the content of interleukin-lβ （IL-1β） in mice brain with enzyme-linked immunosorbent assay （ELISA）,weigh spleen and thymus of mice. Results The effects of different of ingredients Eucommia ulmoides Oliv. on mice sleeping-behavior state varied at the position of hypnotic active time, comparing with positive control.The ratio of sleeping mice was improved and the latent period shortened and the sleeping time prolonged;the levels of IL-1β in mice brain were decreased at different degrees. Conclusion The three kinds of active ingredients had effects on mice falling to sleep and adjusting immunity.Ethyl Acetat （EtOAc） ingredients showed the best effects.
出处
《上海中医药杂志》
北大核心
2006年第11期63-65,共3页
Shanghai Journal of Traditional Chinese Medicine
关键词
杜仲
睡眠
免疫
IL—1β
行为状态
Eucommia ulmoides Oliv.
sleep
immunoregulation
interleukin-1β
behavioral state
