摘要
比较了全骨髓法和密度梯度离心法分离、纯化小鼠骨髓间充质干细胞,并研究了不同培养基和血清对干细胞生长的影响以及小鼠骨髓间充质干细胞的神经分化潜能。结果表明,密度梯度离心法和贴壁法结合使用可以分离纯化小鼠骨髓间充质干细胞;所获得的小鼠骨髓间充质干细胞具有诱导分化为神经元样细胞和脂肪样细胞的潜能,为小鼠骨髓间充质干细胞体外定向诱导分化的研究提供了技术支撑。
The present experiments compared whole-marrow isolation with density gradient isolation for the purification of MSCs, and investigated the influence of different culture media and sera on culture of MSCs, together with their ability of differentiation into neuron-like cells. It was proved that density gradient isolation combined with adherence isolation can purify MSCs, which was able to differentiate into neuron-like cells and adipogenic cells as well. Accordingly it provides a powerful technical support for the luther study of MSCs differentiate into neuron like cells.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2006年第6期696-700,共5页
Chinese Journal of Veterinary Science
基金
山东省自然科学基金(Y2005D01)
教育部重点科研项目(01608)
关键词
小鼠
骨髓间充质干细胞
细胞培养
神经分化
mice
mesenchymal stem cell
cell culture
neuronal differentiation
