摘要
试验随机选取138头迪卡配套系猪B系、216头三江白猪和192头亚洲野猪提取基因组DNA进行聚合酶链式反应,得到RYR_1基因特异片段,经过双酶切鉴定,测得Hal^N基因频率分别为87．31%、92．13%、92．97%；Hal^n的基因频率分别为12．69%、7．87%、7．03%。然后对3种氟烷基因型的猪进行肉质鉴定。
138 DIKERS,216 white pigs, 198 Sus scrofa were selected randomly, selecting blood samples from Sanjang White pigs,the DNA were distilled in blood and purified , by PCR amplified and enzyme digestied , the genotypes of RYR1 was analysised. The results indicated that ： after conditioning optimized by PCR , a fragment of 659 bp was amplified by PCR , the fragment was digested by Hha Ⅰ and HgiA Ⅰ , three genotypes of Hal^N Hal^N , Hal^N Hal^n, Hal^n Hal^n were gained . The frequency of HalN genotype among three species was 87.31% ,92.13% ,92.97% in turn . The frequency of Haln genotype was 12.69%, 7.87% ,7.03%.
出处
《黑龙江畜牧兽医》
CAS
北大核心
2006年第11期19-22,共4页
Heilongjiang Animal Science And veterinary Medicine
基金
黑龙江省科技攻关项目(GB01B105-01)
