摘要
猪应激综合症由氟烷基因型决定,有三种基因型:阳性(nn)、阴性(NN)和杂合子(Nn),nn型产生应激综合症.采用聚合酶链反应(PCR)扩增一段含有突变位点的氟烷基因片段,用限制性内切酶酶切分析,测定杜洛克猪品系37头,有3头为杂合子,34头为阴性.
Porcine stress Syndrome (PSS) is controlled by Halothane gene. The diagnosis of the porcine Halothane gene types was established by polymerase Chain reaction (PCR). The Halothane fragement containing the mutation point was amplified and the PCR product was incubated with the restiction endnuclease HhaI. In total 37 duroc pigs, the gene types of 3 pigs are Nn, the others are NN.
出处
《湖北畜牧兽医》
1998年第2期12-13,共2页
Hubei Journal of Animal and Veterinary Sciences
