摘要
目的:观察环磷腺苷葡胺(M eglum ine Ademosine Cyelophosphate)对其干预作用,并阐述预处理诱导脑缺血耐受的机制。方法:制作大脑中动脉缺血2小时后再灌注损伤模型。SD大鼠54只,随机分为假手术组(SG组)、致死性缺血再灌注组(IR组)、腺苷预处理组(AI组),依术后处死动物时间的不同,每组再分为3个亚组(SG:1、3、5 d,IR:1、3、5 d,AI:1、3、5 d)。结果:采用干湿重法测定脑组织含水量,全自动生化分析仪测定脑组织含钙量。观察预处理对缺血2 h再灌注损伤24 h、72 h、120 h的影响。结果①IR组钙含量术后1、3、5天均高于SG组(P<0.05);AI组各亚组钙含量均低于同期IR组(P<0.05),AI 1 d组钙含量与SG组相比无显著性差异(P>0.05)。②IR组脑组织含水量于再灌注后1、3、5 d均高于SG组(P<0.05);AI组脑组织含水量均低于同期IR组(P<0.05),AI 1 d组脑组织含水量与SG组相比无显著性差异(P>0.05)。结论:①环磷腺苷预处理均能显著减少大鼠局灶性脑缺血后脑组织钙含量;②环磷腺苷预处理还能减轻大鼠局灶性脑缺血后脑水肿。
Objective：To investigate protective effect of meglumine ademosine cyelophosphate in hippocampus of rats following brain ischemia and the mechanism Of neuroprotective effect of negoumine ademosine cyelophosphate. Methods ：2 hours rats model of MCAO（ middle cerebral artery occlusion） was made. 54 rats were randomly divided into the sham operation group （ SG grouop, n = 18）, the ischemia reperfusion group （ IR guoup, n = 18）, and the preconditioning with ademosine group（ AI group, n = 18）. SG , IR and AI groups were further divided into three subgroups respectively （ SG1d, SG3d, SG5d; IR1d, IR3d, IRSd; AI 1d, AI3d, AISd）, with 6 in each subgroup（ n =6）. Brain water content was determined by dry wet weight method. The content of Ca^2＋ was determined by the Dimention RXL. Results： ① The content of Ca^2＋ in the IRld,the IR3d,the IRSd group were significantly higher than that of in the SG group （P 〈 0.05） ;The content of Ca^2＋ in the AI group were significantly lower than those in the IR group（ P 〈 0.05）, and there were no obvious differrence in the content of Ca when the AIld group were contrasted with the SG group（P 〉 0.05）. ② The content of water in brain in the IR group were significantly higher that in the SG group（ P 〈 0.05 ） ;The content of water in the brain in the AI group were lower than those in the IR group （P 〈 0.05）, and there were no obvious change in the content of water when the AI 1d group were contrasted with the SG group（P 〉 0.05）. Conclusions： Meglumine ademosine can signigicantly reduce the content of water and Ca^2＋ in the brain of rats after brain ischemia reperfusion.
出处
《中国民康医学》
2006年第21期931-933,共3页
Medical Journal of Chinese People’s Health
关键词
环磷腺苷
预处理
缺血再灌注
保护作用
Meglumine ademosine cyelophosphate
Precondition ischemic reperfusion
Neuroprotective effect
