摘要
禾本科岩生植物金发草〔Pogonatherum paniceum(Lam.)Hack.〕具有开发为新型国产草坪草种的潜力.用IS-SR与AFLP分子标记,分析了四川、重庆、云南及广西等地22个金发草种群的遗传多样性.用14个ISSR引物、10对AFLP引物分别扩增出239、485条谱带,多态位点百分率分别为94.98%、90.93%.两种标记均揭示出金发草种群具有丰富的遗传多样水平.由于标记原理不同,两种标记的结果呈现出微小的差别.相对而言,基于ISSR标记分析的遗传多样性参数高于AFLP标记得到的遗传多样性参数,这可能是由于金发草种群间存在着大量的微卫星变异所致.两种标记基于Nei遗传距离的聚类分析结果存在着一定的差异,但用Mantel测试对两种方法检测的遗传一致度进行相关性分析表明,它们之间存在着显著的相关性(r=0.3146,P=0.0150),用SPSS11.5软件进一步分析两种标记遗传一致度的相关性,Pearson相关系数为0.315(在0.01水平上),两种标记的聚类分析都揭示出金发草的遗传变异呈现出一定的地域分布规律.ISSR标记与AFLP标记均能应用于金发草种群的遗传多样性研究.
As a rock plant, Pogonatherum paniceum （ Lam. ） Hack. is a promising new native turfgrass species for gardens in China. Its genetic diversity of 22 populations from Sichuan, Yunnan, Chongqing and Guangxi was analyzed using inter-simple sequence repeats （ISSR） markers and amplified fragment length polymorphism （AFLP）. Fourteen ISSR primers and ten AFLP primer combinations amplified 239 and 485 polymorphic bands, of which the polymorphic loci were 227 （94.98%） and 441 （90.93%）, respectively. Similar results of ISSR and AFLP revealed that P. paniceum possessed higher genetic diversity. However, because of the different mechanisms of ISSR and AFLP, minor difference existed in the results obtained. Genetic diversity parameters based on ISSR marker were higher than those based on AFLP, which was probably resulted from the abun-dant variations of microsatellites occurred in inter-populations. Although some differences existed between the ISSR and AFLP UPGMA dendrograms based on Nei＇s genetic distance, Mantel test revealed significant correlation of genetic identity matrix between ISSR and AFLP （r = 0. 314 6, P = 0. 015 0） in the 22 populations, which was further confirmed by Pearson correlation coefficient based on SPSS11. 5 software （0.315, above the level of 0.01 ）. ISSR and AFLP markers revealed the genetic varia- tion of P. paniceum presented a geographical distribution pattern. Both markers could be used as tools for analyzing the genetic diversity of P. paniceum.
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2006年第5期605-608,共4页
Chinese Journal of Applied and Environmental Biology
基金
教育部973预研项目(104255)资助~~
