摘要
目的通过研究慢性丙型肝炎患者精液中丙型肝炎病毒感染,探讨丙型肝炎病毒是否经夫妻性生活途径传播。方法用酶联免疫吸附法和荧光定量PCR法,检测56例男性慢性丙型肝炎患者血清、精液及其54例配偶血清抗-HCV、HCVRNA,健康对照组为23对体检健康者夫妇。结果男性慢性丙型肝炎患者血清中抗-HCV、HCVRNA的阳性率分别为91.07%(51/56)、85.71%(48/56),精液中抗-HCV、HCVRNA的阳性率分别为39.28%(22/56)、62.50%(35/56),结果与健康对照组比较均有统计学意义,P<0.05;男性慢性丙型肝炎患者配偶的血清抗-HCV及HCVRNA的阳性率为22.22%(12/54)、24.07%(13/54),与健康对照组相比较,经χ2检验有统计学意义,P<0.05。结论慢性丙型肝炎患者精液可感染HCV;HCV可经夫妻性生活途径传播。
Objective By studying hepatitis C virus （HCV）in semen of chronic hepatitis C （CHC） cases to explore whether the hepatitis C virus could be transmitted by means of sexual activities of spouses. Methods The anti-HCV was monitored by indirect enzyme-linked immunosorbent assay（ELISA） and the serum levels of HCV RNA quantities were tested by fluorescent quantitative PCR （FQ-PCR）in 56 cases sera and semen of male chronic hepatitis C patients,and the anti-HCV and HCV RNA were detected by the same methods as above in 54 sera of CHC＇s wives. 23 health couples were as the control group. Results 91.07% （51/56）and 85. 71%（48/56）was belonged to anti-HCV and HCV RNA in sera of 56 CHC cases,respectively,and 39.28% （22/56）and 62. 50%（35/56）of antiHCV and HCV RNA was carried out in semen of 56 CHC cases,respectively. There was a statistisal signincance between exam group and control group by X^2 rtested, P〈0.05.22.22% （12/54）and 24. 07%（13/54）of anti-HCV and HCV RNA was detected in sera of CHC＇s wives. There was a statistisal signincance compare with the control group by .）X^2 tested,P〈0.05. Conclusion The semen of chronic hepatitis C can be infected hepatitis C virus. Hepatitis C virus can be transmitted by means of sexual activities of spouses.
出处
《国际检验医学杂志》
CAS
2006年第10期874-875,878,共3页
International Journal of Laboratory Medicine
关键词
丙型肝炎
慢性
肝炎病毒
精液
性传播
聚合酶链反应
Hepatitis C chronic
Hepacivirus
Semen
Sexual transmission
Polymerase chain reaction
