摘要
本实验采用SD大鼠56只,于顶皮质作一宽2mm的冠状切口,用尼氏染色和AchE阳性纤维染色,观察损伤后胰岛素对切口两侧胶质细胞变化的影响.结果:损伤组和损伤用药组损伤切口两侧的胶质细胞在1～2W内迅速增生和集聚;2～3W出现胶质瘢痕,4～8W内增生的胶质细胞减少,胶质瘢痕收缩成一条致密带,再生胆碱能纤维沿切口两侧生长,难以穿越瘢痕组织,胰岛素用药组皮质损伤切口两侧的胶质细胞增生虽较损伤组轻,但还不足以有效的抑制胶质瘢痕的形成.
56 SD rats(two month old) were operated wtih a incision at the parietal cortex. Nissl and AchE staining methods were used for observing the effect of insulin on the changes of glia and cholinergic fibers. The results showed that as follow: in both the damage rats and the damage rats with insulin injection , the number of the glial cells at the both side of the incision increase rapidly in 1~2 weeks after lesion. And the glial scar appears in 3~4 weeks. However, the num-bet of the glial cells decrease in 4-8 weeks . A band of glial scar forms at this time and the AchE fibers can not pass through the glial scar. Although insulin can reduce the glial reaction after cortex damage, it can not stop the formation of glial scar.
