摘要
从各种海泥分离纯化到多株海洋嗜盐细菌,经筛选发现其中F-5菌株等属于年盐杆菌属(Halobacterium),能产生胡萝卜素。在经选定的最适条件下发酵时,其所产生的胡萝卜素经提取和纯化,获得紫红色的结晶,通过现代波谱技术,包括紫外和红外吸收光谱、质谱、质子和碳-13-磁共振谱的鉴定,确认是β-胡萝卜素。除F-5菌株外,还有一些待鉴定的菌株也能产生胡萝卜素。这是嗜盐细菌能产生β-胡萝卜素的首例报告。
Strain F-5 and several other strains of Halobacteria were isolated from saltern pool of Xiamen, China. Strain F-5 is Gram-negative rod and obligate aerob'e. Optimum salt concentration for strain F-5 growth is 18% to 26% NaCl and no growth below 14% NaCl. And strain F-5 can produce carotenoids pigments. Therefore, strain F-5 is considered as one member of extreme Halogacteria.
Natural pigment F-5-C1 is mainly produced by strain F-5. F-5-C1 is obtained as a purple crystal and soluble in organic solvents, but no soluble in water. F5-C1 exhibits a molecular ion by EI-MS at m/z 536. 9, corresponding to the formula C40H56. The UV and IR spectra of F-5-C1 is identical with that of fj-carotene (E. Merck product), which was isolated from carrot. These informations coupled with 'HNMR, 13CNMR and MS spectral data allowed us to consider that F-5-C1 is identified with B-carotene. And this is the first report on B-carotene produced by one strain of Halobacteria.
出处
《中国海洋药物》
CAS
CSCD
1996年第4期1-4,共4页
Chinese Journal of Marine Drugs
基金
福建省自然科学基金(编号C96061)
