摘要
通过测定不同基质浓度(C+N=0.001g/mL、0.005g/mL、0.015g/mL、0.045g/mL、0.135g/mL)对淡紫拟青霉“NH-PL-03”菌株生长的影响结果表明,基质浓度对菌体生长有明显影响,基质浓度为0.045g/mL时菌丝生长旺盛,培养10d每瓶干物质量达到0.82g,对茎线虫校正致死率在8d达到峰值52.30%。基质浓度为0.015g/mL的实验组合产孢量最大,培养8d产孢量达峰值69.20百万个/mL。0.135g/mL的基质浓度对菌生长表现出明显抑制作用,培养10d其干物质量和产孢量仅分别为0.23g和15.10百万个/mL。
The effect of different substrate concentrations on the growth of Paecilomyces lilacinus ＂NH-PL-03＂was studied. The results show that the substrate concentration has a significant effect on the growth of Paecilomyces lilacinus. To some degrees, a lower substrate concentration （C ＋ N = 0.015g/mL） is good for sporulation, the sporulation reaches the peak value of 69.20 million/mL at 8d, and a higher concentration （C ＋ N = 0.045g/mL） benefits the biomass growth and toxic outcome, the maximal dry matter weight of culture liquid of 0.82g（each 50mL） occurs at 10d, the toxic result to Ditylenchus destructor is 52.30 % at 8d. The highest substrate concentration （C ＋ N = 0. 135g/mL） shows obvious inhibitting effect on the growth of Paecilomyces lilacinus, whose dry matter weight and sporulation are 0.23g and 15.10 million/mL at 10d.
出处
《中国生态农业学报》
CAS
CSCD
2006年第4期153-156,共4页
Chinese Journal of Eco-Agriculture
基金
国家高技术研究(863)发展计划项目(2002AA244031-2)
福建省计委项目(2003-170)资助
关键词
淡紫拟青霉
基质浓度
菌液干物质量
产孢量
Paecilomyces lilacinus, Substrate concentration, Dry matter weight of culture liquid, Sporulation
