摘要
跟踪检测了淡紫拟青霉NH PL 03菌株液体培养过程中D(620nm)值、pH值、菌丝干重、产孢量以及基质消耗 (碳、氮)等培养参数,对培养因子进行了相关分析和主成分分析,并对该菌株的培养阶段进行了聚类分析.结果表明:菌丝干重、产孢量、D(620nm)值分别与营养生长、生殖生长、生理特性主成分;基质消耗 (C、N)与产物生成呈显著相关;当λ=2. 582时,可将淡紫拟青霉菌株NH PL 03培养过程分为 4个阶段,第 1阶段为适应期,培养时间为 1 -2d,菌的培养参数变化不大;第 2阶段为生长期,培养时间为 3-5d,菌体开始生长,D(620nm)值上升,pH值下降,菌丝和产孢量增加;第 3阶段为对数期,培养时间为 6-7d,D(620nm)值、pH值上升,菌丝和产孢量急剧增加; 8d后进入稳定期 (第 4阶段 ),D(620nm)值、pH值趋于稳定,菌丝干重和产孢量达到峰值并趋于稳定.
Growing indexes of Paecilomyces lilacinus (Thom.) Samson strain NH-PL-03 such as D(620 nm) value, pH value, dry weight of mycelia, sporulation and the C, N consuming in the liquid culturing process had been systemically investigated. The results were as follows: (1) the rhythm growing were interrelated to C and N consuming significantly. (2) dry weight of mycelia, sporulation and D(620 nm) value were the principal components of nutritious growth, reproduction and physiological status, respectively. (3) based on the cluster analysis, when λ was equal to 2.582 the cultural process can be divided into four stages: the first one (1-2 d) was an adaptation stage, with all growing indexes gradually changing; the second one(3-5 d) was a growth stage, all growing indexes gained in rapidly, biomass increased significantly; the third one (6-7 d) was a logarithmic stage, all growing indexes leaped forward and the biomass increased rapidly; the fourth one (after 8 d) was a stagnant stage, all growing index was stable relatively.
出处
《福建农林大学学报（自然科学版）》
CSCD
北大核心
2005年第1期25-29,共5页
Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金
国家高新技术研究发展计划(2002AA244031 2).
