摘要
家蚕反转期胚胎组织经过一年多的原代培养,建立了BmE-SWU2细胞系。该细胞系细胞呈短梭形或圆形,细胞较小,属上皮型贴壁生长细胞系;细胞生长速度快,铺平率高于80%,繁殖力强,细胞群体倍增时间为51·8h。BmE-SWU2细胞系的二倍体细胞(2n=56)比例达到89·76%,属二倍体细胞系,其染色体具有鳞翅目昆虫染色体的典型特征,中期染色体呈短杆状或颗粒状。BmE-SWU2细胞系对BmNPV高度敏感,TCID50为1·581×10-7。
An embryo cell line of Bombyx mori, named BmE-SWU2, was established after one year primary culture. The cells are relatively single, short shuttle-like or round, and very small in shape. The cell line belongs to adhesive-type epithelium. The cell doubling time was 51.8 h. The ratio of duple cells was 89.76%. Thus, the cell line belongs to duple cell line. The chromosomes of BmE-SWU2 was typical of Lepidoptera, namely the chromosomes in metaphase were short pole-like or granule-like and the centromeres dispersed-type. BmE-SWU2 was more sensitive to BmNPV. The 50% tissue culture infection dose （TCID50） was 1.581×10^-7.
出处
《昆虫学报》
CAS
CSCD
北大核心
2006年第5期719-725,共7页
Acta Entomologica Sinica
基金
国家"973"项目(2005CB121000)
国家自然科学基金项目(30471312)
国家科技攻关计划(2005BA711A07)
关键词
家蚕
胚胎细胞系
核型
生长曲线
病毒敏感性
Bombyx moti
embryo cell line
karyotype
growth curve
vires susceptivity
