摘要
背景与目的：细胞凋亡调控失衡在肿瘤发生发展中具有重要意义。Survivin、Caspase3是细胞凋亡和细胞周期调控的重要成员。本研究应用组织芯片技术分析胃癌组织Survivin、Caspase3等的表达与胃癌临床病理特征和生物学预后的相关性及意义；方法：将100例无术前放化疗史的胃癌手术标本和30例对照组胃粘膜制成组织芯片，取样针直径2．0mm。用免疫组化方法分别检测Survivin、Caspase3等的表达，用TUNEL法进行细胞凋亡指数检测，对随访14个月～13年的47例作生存分析。结果：共获得2个组织芯片蜡块，分别含114和116个位点：组织芯片中，Survivin主要在胃癌中表达（78％），而对照组胃粘膜几乎无表达（P〈0．01），Survivin阳性率与胃癌组织学分化、淋巴结转移、血管／神经侵犯（BV／N）及TNM分期相关（P〈0．05）；Caspase3在正常粘膜的表达（90％）显著高于胃癌组织（56％）（P〈0．01），阳性率与胃癌组织学分化、BV／N相关（P〈0．05），而与淋巴结转移和TNM分期无关（P〉0．05）。Survivin阳性组细胞凋亡指数明显低于阴性组（P〈0．01），而Caspase3阳性组凋亡指数明显高于Caspase3阴性组（P〈0．05）：在有随访的47例中，单因素Cox分析显示，BV／N、TNM分期及Survivin表达均与预后相关，而组织学分化、Caspase3表达与预后无关，经多因素Cox分析显示，只有TNM分期、Survivin表达具有独立的预后意义。结论：Survivin表达与胃癌临床病理特征及预后密切相关，似可作为胃癌预后差的指标，Caspase3可能参与细胞凋亡调控和胃癌生物学进程.
Background and purpose： Unbalance of apoptosis regulation plays an important role in tumor initiation and progression. Survivin and easpase-3 all take part in the regulation of cell cycle and apoptosis. We investigated the role of survivin and easpase-3 in apoptosis of gastric carcinoma, as well as its relationship with the clinicopathologic features and the prognosis of gastric carcinoma by using tissue mieroarray. Methods： The gastric cancer tissue mieroarrays were composed of tissues from 100 cases of carcinoma of the stomach without a history of ehemo-radiation therapy and 30 gastric tissues of control subject. The diameter of each specimen on tissue mieroarrays was 2.0 mm. Expressions of survivin, easpase-3 were investigated immunohistoehemieally on these tissue mieroarrays. Tumor cell apoptosis index was also examined by TUNEL method. Of the 100 cases, 47 cases were followed-up from 14 months to 13 years, in which the prognosis was analyzed. Results： Two paraffin-embedded gastric carcinoma tissue mieroarrays were successfully constructed, including 114 and 116 tissue spots, respectively. Immunohistoehemical analysis showed that survivin expression was positive in 78 cases （78%） in this series. In contrast, no expression of survivin in control tissues was detected （P 〈0.01）. The expression of survivin was associated with histological grades, lymph node involvement, invading of vessel ＆ nerve（BV/N） and TNM stages（ P 〈 0.05）. The expression of caspase-3 was much higher in normal tissues （90%） than in carcinoma （56%） （P 〈0.01）. Caspase-3 expression was significantly related to histological grades and BV/N, but unrelated to lymph node involvement and TNM stages. The apoptosis index was lower in survivin（ ＋ ） group（3.16 ± 1.03） than in survivin（-） group （4.01 ± 1. 08） （P 〈 0.01）. The apoptosis index was higher in easpase-3（ ＋ ） group than in caspase-3 （-） group （P 〈 0.01）. In the 47 cases with followed-up data, univariant analysis
出处
《中国癌症杂志》
CAS
CSCD
2006年第10期817-822,共6页
China Oncology
