摘要
在比较不同的提取方法后,选用乙醇为提取溶剂。通过单因素实验结合响应面法对白首乌中抗氧化成分的提取工艺进行优化研究,确定了提取工艺的最佳条件为温度:70.1℃,乙醇体积分数:75%,提取时间:1.78 h,提取次数为两次。在此条件下白首乌醇提取液的总抗氧化能力理论值为158.68 U/g,实测值为164.07U/g±3.73。
Based on the results of single-factor-at-a-time-technique, a three-level Box-Behnken factorial design combining with response surface methodology（RSM） was employed to optimize the extraction condition of Cynanchum auriculatum Royle. Canonical analysis was applied to estimate that a maximal yield of total antioxidant capacity （TAC）（158. 68 U/g） could be obtained. When with two-time extraction, the extraction conditions, such as temperature, extraction time and the concentration of ethanol was 70.1℃ ,75% and 1.78h,respectively. These predicted values were also verified by validation experiments.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2006年第5期81-87,共7页
Journal of Food Science and Biotechnology
关键词
白首乌
总抗氧化能力
响应面法
BOX-BEHNKEN设计
Cynanchum auriculatum Royle ex Wight
Total antioxidant＇ capacity
Response surface methodology
Box-Behnken design
