摘要
目的探索TD-RAPD技术用于罂粟品种鉴定的可行性。方法采用改良CTAB法从罂粟叶片中提取DNA;TD-RAPD技术对种植于云南西双版纳地区的1个罂粟样品进行扩增分析。结果建立了罂粟DNA提取方法,从10个随机引物中筛选出6个引物用于罂粟TD-RAPD分析。结论TD-RAPD技术可用于罂粟DNA的分子标记,为罂粟DNA数据库建立提供技术方法,最终从DNA分子水平上追溯罂粟植物毒源。
Objective The feasibility of Papaver somniferum L. cuhivars identification was explored by TD-RAPD technique. Method Genomic DNA was extracted by improved CTAB method. One sample of species from Papaver somniferum L in xishuangbanna area. was studied by using TD-RAPD method. Result We established an optimal method of extracting genomic DNA. Six primers were picked out from 10 primers. Conclusion TD-RAPD could be applied to researches of molecular marker of Papaver somniferum L. TD-RAPD technique provide a method to constitute DNA database of Papaver somniferum L. and conclude the source of opium poppy.
出处
《法医学杂志》
CAS
CSCD
2006年第5期367-369,共3页
Journal of Forensic Medicine
