摘要
在丝网印刷碳糊电极上利用吸附法固定葡萄糖氧化酶或尿酸酶，并用碳纳米管进行修饰，铁氰化钾作为电子传递剂，制作用于测量人体血浆中葡萄糖和尿酸浓度的生物传感器．葡萄糖传感器的响应时间仅为5s，响应电流范围为1．2～30μA，线性测量范围为1～33．3mM，尿酸传感器响应时间为和电流范围分别为50s，0．7～14μA，线形测量范围是2～20mg／dL．用碳纳米管修饰酶电极，改善了电极表面条件，加快了电极反应速度，并提高了传感器的灵敏度．通过碳纳米管修饰电极，葡萄糖传感器的灵敏度从0．3338μA／mM提高到0．8432μA／mM，尿酸传感器的灵敏度从0．4028μA／（mg／dL）提高到0．7138μA／（mg／dL）．
The biosensors are prepared by immobilizing glucose oxidase and uricase respectively on the surface of screen-printed carbon paste electrodes modified by carbon nanotubes, while potassium ferricyanide is employed as electron transfer mediator. The glucose biosensors＇ response time is only 5 s while the response current is 1.2-30 μA for measurable linear range of 1-33.3 mM. The response time and current of the uric acid biosensors are 50 s and 0.7-14 μA for 2-20 mg/dL, respectively. The comparison experimental results show that the carbon nanotube modification speeds up the electrode reaction and improves the sensitivities of the glucose and uric acid sensors. The sensitivity of the glucose sensors is increased from 0. 333 8 to 0. 843 2 μA/mM by the carbon nanotube modification, while the sensitivity of the uric acid sensors is increased from 0. 402 8 to 0. 713 8 μA/（mg/dL）.
出处
《传感技术学报》
CAS
CSCD
北大核心
2006年第05B期2077-2080,2083,共5页
Chinese Journal of Sensors and Actuators
基金
国家自然科学基金资助(60421002)
