摘要
对安哥拉山羊用总量３５０ＩＵＦＳＨ按递减法处理，配种结束后第６天经子宫手术回收桑椹胚和囊胚供冷冻试验。选用１．４ｍｏｌ／Ｌ甘油、１．２ｍｏｌ／Ｌ和１．５ｍｏｌ／Ｌ乙二醇做防冻保护剂，快速冷冻，－３６℃投入液氮保存。采用直接水浴或在空气中停留６～１０ｓ使胚胎温度回升，再水浴解冻。胚胎解冻后，用０．５ｍｏｌ／Ｌ蔗糖ＰＢＳＳ脱除保护剂，移入２％ＰＢＳＳ中，手术移植于发情后６ｄ的适移受体。结果表明，１．５ｍｏｌ／Ｌ乙二醇具有良好的冷冻保护作用。在１８～２０℃空气中使胚胎温度稍回升，再水浴解冻，可降低胚胎解冻过程中热应激导致的透明带破损，移植于适移受体的妊娠率达４５．５％
Embryo freezing of Angora goats were studied. Morulae and blastocysts were obtained surgically from uteri on day 6 postmating. Cryoprotectant solutions were 1.4 mol/L glycerol, 1.2 mol/L ethylene glycol (EG) or 1.5 mol/L EG in phosphate buffered saline (PBS) containing 20% new born calf serum (NCS). The quick freezing method was used, the embryo straws were plunged into liquid nitrogen at -36℃. Embryo were direct thawed in 32℃ water bath for 15 seconds or were slowly warmed in 18￣22℃ air for 6￣10 seconds prior to transfer into water. Removed of the cryoprotectant solution were carried out by placing thawed embryos in PBS containing 0.5 mol/L sucrose and supplemented with 2% NCS, then embryos were placed into PBS with 2% NCS and were transfered surgically into suitable recipients which were day 6 post estrus. The results of this study indicated that cryoprotectant effect of the 1.5 mol/L EG was better than others. Below 18￣22℃, embroys were slowly warmed, then thawed in water bath, zonae damage resulted in thermal stress was reduced, pregnancy rate of transferring to suitable recipients was 45.5%.
出处
《西北农业学报》
CSCD
1996年第4期36-40,共5页
Acta Agriculturae Boreali-occidentalis Sinica
基金
国家"八五"重点科技攻关项目
