摘要
本实验对小鼠裸半胚采取了多种不同的处理方式，其中冷冻前不培养、冷冻前经短时间培养和冷冻前经长时间培养的冷冻半胚解冻后培养发育率分别为１４．５５％、２６．６７％和６．１２％；装透明带半胚和装透明带后再用琼脂包埋半胚的解冻后培养发育率分别为５６．６７％和６４．２９％；小鼠半胚快速冷冻解冻的培养发育率为６２．００％。此外．将１０枚奶牛半胚装带、包埋、冷冻解冻后，移植给５头受体．结果有１头妊娠；奶牛整胚冷冻后分割的半胚移植妊娠率为２８．５７％（４／１４）。
The naked mouse demi-embryos incubating for 0 h, 2-4 h. and 20-24 hrs before deepfreezing were compared. The developing rate of these when in cubated in PBS containing20% FCS or sheep serum and humid air at 38℃ for 48 h was 14.55% (8/55), 26.67%(12/45) and 6.12% (3/49), respectively. The developmtneal rate of the frozen defrosteddemi-embryos with zona pellucida and demi-embryos with zona pellucida embedded inagar in vitro culture were 56.67% (17 / 30) and 64.29% (18 / 28). The developing rate ofmouse demi-embryos with zona pellucida in vitro culture after guick freezing defrosting inPBS solution containing 3.5 M glycerol and 0.5 M sucrose was 62.00% (31 / 50). Tenbovine demi-embryos with zona pellucida and embedded in agar were frozen with ordinaryway, and transferred to five recipient cows after defrosting, two demi--embryos per recipient. Pregnancy diagnosis revealed one pregnant animal. The pregnant rate of transferredbovine demi-embryos, bisected after freezing the whole embryos, was 28.56% (4 / 14).
出处
《山东农业大学学报（自然科学版）》
CSCD
1994年第3期332-334,共3页
Journal of Shandong Agricultural University：Natural Science Edition
