摘要
目的:建立HPLC法测定青钢注射液中绿原酸的含量。方法:采用C18(180×4·6mmI.D10um)色谱柱,甲醇-水(0·2mol/L.调pH为3·2)(15+85)为流动相,流速1ml/min,检测波长328nm。结果:青银注射液中绿原酸的含量为2·227±0·0414mg/ml,绿原酸线性关系为r=0·9999,平均回收率99·75%,rsd为3·51%。结论:此方法分离效果较好,方法可靠并能确保产品质量。
Objective： To establish a new method for determination of chlorogenic acid in the Qingyin injection by HPLC. Methods： Chromatographic conditions were column： C18 （180×4.6 mmⅠ. D 10 um） mobilephase, MeOH-H20 （0.2 mol/L. pH = 3.2） （15：85） flowratelml/min, detector wave length： 328 nm. Results： The content of chlorogenic acid in the Qingyin in jection is 2.227± 0.0414 mg/ml, the correlation coefficient was 0.9999, and average recovery （ASR） was 99.75%, RSD = 3.51%. Conclusion： This method have good separate effect and is credible enough to insure the quality of products.
出处
《现代预防医学》
CAS
北大核心
2006年第10期1943-1943,1945,共2页
Modern Preventive Medicine
