摘要
目的评价早期雌激素替代治疗（ERT）对去势0～20d大鼠松质骨组织形态扣骨密度的作用。方法40只4月龄雌性SD大鼠随机分成5组，除第1组行假手术外，其余均行双侧卵巢切除，第1、2组予安慰剂处理，其余3组分剐在术后0、10和20d以尼尔雌醇1．5mg／（kg·周）灌胃。8周后处死取股骨侧其近中远段骨密度扣胫骨干骺端行组织形态学测量。结果去势对照组在股骨骨密度、骨小梁面积百分率、骨小梁间距、骨小梁厚度扣节点数等多个指标上扣正常对照组存在显著性差异（P〈0．01）；术后即行ERT者在各个指标中均扣正常组无明显区别，延迟10d后治疗组骨密度、骨小梁面积百分率和正常对照组无统计差异，20d后开始ERT组仅部分恢复骨密度和骨小梁面积百分率，后两组骨小梁连接性均有明显退变。结论ERT能抑制扣恢复骨量的丢失，但不能逆转骨小梁三维结构的退变，早期行ERT是防止绝经后骨质疏松的主要方法。
Objective To evaluate the ability of estrogen replacement therapy（ERT） to prevent changes in trabecular bone volume（TBV）, bone mineral density（BMD） and connectivity begininning either at ovariectomy（OVX） or 10～20 days after OVX in adult female rats. Methods 40 rats was divided into 5 groups, groups 2～5 had bilateral OVX, while groupl was sham-ovariectomied on day 0. Animal were treat with vehicle （groupsl and 2） or ERT starting at days 0, 10, 20 post-ovx （groups 3～5）, 8 weeks later they were sacrificed, trabecular variables were calculated from histomorphometry and DEXA. Results Animal given ERT from the time of OVX maintain the BMD, TBV and trabecular connectivity, however, OVX animal treat with vihicle deteriorated greatly, ERT innitiated at day 10 and 20 restored BMD and TBV partly, but the trabecular connectivity were not restored. Conclusion ERT can restore the lost bone, but cannot restore the trabecular connectivity.
出处
《四川医学》
CAS
2006年第10期1002-1004,共3页
Sichuan Medical Journal
