摘要
以L-苏氨酸生产菌TRFC为供试菌株,研究了碳源对L-苏氨酸发酵的产量和糖酸转化率的影响。采用补料分批发酵工艺生产L-苏氨酸,利用氨基酸分析仪测定发酵液中L-苏氨酸的产量。确定了发酵的最佳碳源及其补料方式,通过10L罐补料分批发酵36h,产酸可达118.9 g/L,糖酸转化率为47.6%。
The effects of carbon source on the concentration of L - threonine and conversion rate of glucose were studied with L - threonine production strain TRFC. L - threonine was produced by fed - batch fermention styles, L - threonine fermentation concentration in fermentation broth was determined with the methods of amino acid analysis system. The optimum carbon source and its feeding mode were determined. The productivity of L - threonine was 118.9 g/L in 10- liter fermentor at 36 h, the conversion rate of glucose was 47.6%.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2006年第8期15-17,共3页
Food and Fermentation Industries
关键词
L-苏氨酸
碳源
补料分批发酵
L - threonine, carbon source, fed- batch fermentation
