摘要
目的:采用高效液相色谱法测定环磷腺苷针剂的含量及有关物质。方法:采用 ODS C_(18)色谱柱(4.6 mm×150 mm,5μm),以甲醇-0.025 mol·L^(-1)磷酸二氢钠缓冲液(二水合磷酸二氢钠1.95g 加水至500 mL,用磷酸调 pH 至5.0)(10:90)为流动相,流速0.8 mL·min^(-1),于254nm 测定。结果:HPLC 法测定的线性范围为0.04～0.4 mg·mL^(-1),r=0.9999,最低检测限0.24 ng,对照品溶液在8 h 内稳定,日内和日间精密度(RSD<1.2%)良好。回收率为99.87%。结论:采用 HPLC 法测定环磷腺苷及有关物质的方法简便,结果可靠。
Objective：To determine the content and the related substances of adenosine cyclophosphate by HPLC Methods： The column ： ODS C18 column （4.6 mm × 150 mm,5 μm） ; The mobile phase ： methanol - NaH2 PO4 buffer （0. 025 mol 2 L^-1monobasic sodium phosphate,adjust pH to 5.0 by phosphoric acid） （ 10： 90） ;The flow rate：0. 8 mL · min^- 1 ;The content of adenosine cyclophosphate and its related substances were detected at 254 nm on the same condition. Results ： The linear range of the determination by HPLC was 0. 04 - 0. 4 mg · mL ^- 1 ; The correlation coefficient was 0. 9999 ; The least detective range was 0. 24 ng; The sample solution was stable within 8 h ; The precision of intra - day and inter - day were good （ RSD 〈 1.2% ）. The recovery result was 99.87%. Conclusion ： The method to determine the content and related substances of adenosine cyclophosphate by HPLC is easy to operate and the results of the determination are accurate.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2006年第8期1121-1123,共3页
Chinese Journal of Pharmaceutical Analysis
基金
陕西省教委基金项目(03JK075)
陕西省自然科学基金(2005B18)
