摘要
目的了解谷胱甘肽硫转移酶A1(GSTA1)基因多态性在中国南方客家人群中的分布规律。方法采用聚合酶链式反应-限制性酶切片段长度多态性(PCR-RFLP)方法检测广东省梅州地区480名客家健康居民GSTA1基因型,应用SPSS10·0统计软件进行统计分析。结果调查人群GSTA1*A/*A、GSTA1*A/*B、GSTA1*B/*B三种基因型的分布频率分别为77·1%、21·7%和1·2%,三种基因型的分布符合哈迪-温伯格(Hardy-Weinberg)平衡吻合度定律。在不同年龄、性别、生活习惯人群中分布差异无显著性,二分类Logistic回归分析结果显示,高血压、冠心病、脑卒中、肺癌及鼻咽癌等家族史在人群中的发生与人群GSTA1不同基因型的分布无显著相关关系。结论中国南方客家人群GSTA1基因型分布呈现多态性分布,符合哈迪-温伯格定律。
Objective Glutathione-S-transferase A1 （GSTA1） is one of the major phase Ⅱ detoxifieation enzymes in the cytosol, which genetic polymorphisms distribution is different in different ethnic, national and regional population. Up to now, GSTA1 genetic polymorphisms has been rarely reported in China. The purpose of the study was to investigate the distribution of genetic polymorphisms of human GSTA1 in Hakka population in South China. Methods Polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）were used to identify the genotypes of GSTA1 gene and the data were analyzed with SPSS10.0 software. Results The GSTA1 genetic polymorphisms were detected in 480 samples. The frenqueney of GSTA1 ＊ A/＊ A,GSTA1 ＊ A/＊ B and GSTA1 ＊ B/＊ B were 77.1% ,21.7% and 1.2% respectively. And the GSTA1 genetic polymorphism distribution was in accordance with the Hardy-Weinberg equilibrium rule. There were no difference in the GSTA1 genetic polymorphisms among the different groups of age or gender. Logistic regression analysis showed that there were no association between the GSTA1 genetic polymorphism and family history of hypertension, coronary heart disease, stroke, lung cancer and nasophary negeal cancer, et al. Conclusion The GSTA1 gene existed polymorphism among Hakka in South China.
出处
《卫生研究》
CAS
CSCD
北大核心
2006年第5期618-621,共4页
Journal of Hygiene Research
基金
广东省自然科学基金团队项目(No.015042)
