摘要
目的:观察旋磁场对大鼠急性局灶性脑缺血再灌注的影响。方法:实验于2003-03在中国医科大学完成。选择18~20周鼠龄健康Wistar大鼠70只,随机数字表法分成3组,假手术组12只、对照组20只、治疗组38只。治疗组按照再灌注时间又分为4个时间点:再灌注即刻6只,再灌注6h20只,再灌注12h6只,再灌注18h6只。以改良Longa线栓法制备动物局灶性脑缺血模型,假手术组手术操作过程相同,但栓线插入深度为10mm。采用5级评分法评定神经功能缺损来筛选病例。评分1级和2级且缺血2h再灌注24h后存活者入选对照组和治疗组。假手术组和对照组不予旋磁治疗。治疗组治疗时将磁头对准大鼠头部,磁头与皮肤距离7mm左右,治疗时间为15min。各组动物均于再灌注24h时断头取脑。脑含水量采用干湿重法,梗死灶体积采用TTC染色法测定,并分析缺血侧脑组织的超氧化物歧化酶和丙二醛含量及脑组织形态学变化。结果:实验中剔除死亡及模型制备不合格筛选后符合条件动物70只,全部进入结果分析。①治疗组除再灌注即刻时间点外其余各时间点大鼠脑含水量比对照组减少,脑水肿程度均减轻[(2.48±0.22)%,(2.32±0.19)%,(2.23±0.36)%,(2.91±0.44)%,P<0.05]。②治疗组绝对脑梗死体积比对照组减小[(128.21±15.05),(171.22±40.50)mm3,t=2.438,P<0.05],相对脑梗死体积比对照组减小[(20.22±1.44)%,(25.17±3.85)%,t=2.95,P<0.05]。③与对照组比较,旋磁治疗组大鼠脑组织中超氧化物歧化酶含量增加[(54.54±3.85),(69.52±5.88)NU/mg,t=5.568,P<0.05],而丙二醛含量下降[(0.85±0.06),(1.03±0.09)μmol/g,t=4.076,P<0.05]。④大体形态学表现为对照组右侧大脑半球水肿明显,颜色呈脂肪样苍白,脑沟变浅,脑回变平,组织较脆且易碎。而治疗组右侧大脑半球水肿程度较轻,大脑表面充血明显。结论:旋磁场对大鼠局灶性脑缺血再灌注损伤具有预防和治疗作用,此作用可能与减�
AIM: To investigate the effects of rotating magnetic field (RMF) on the acute focal cerebral ischemia/reperfusion.
METHODS: The experiment was conducted at the China Medical University in March 2003. Totally 70 healthy Wistar rats aged 18-20 weeks were selected and randomly assigned into 3 groups: sham operation group with 12 rats, control group with 20 rats and treatment group with 38 rats. The treatment group included 4 time points: immediate reperfusion with 6 ones, 6-hour reperfusion with 20 ones, 12-hour reperfusion with 6 ones and 18- hour reperfusion with 6 rats. Focal cerebral ischemic animal models were established with modified Longa occlusion. Operation was the same in the sham operation, but the thread was inserted as 10 mm. Neurologic impairment was assessed with 5-rating method to screen out cases. Those survivals with grade 1 and grade 2 after ischemia for 2 hours and reperfusion for 24 hours were included in the control group and treatment group. Those in the sham operation group and control group were not treated with RMF. Magnetic head was directed towards the head of rats of the treatment group, and the magnetic head was about 7 mm from skin, treated for 15 minutes. The rats were decapitated to take out brains at hour 24 after reperfusion in each group. Water content of brain and volume of cerebral infarction were assessed with wet-dry weight method and TIC staining, respectively. Activity of superoxide dismutase (SOD), content of malondialdehyde (MDA) and change of brain histomorphology in brain tissue of ischemic side were analyzed. RESULTS: Totally 70 qualified animals were involved in the result anal- ysis after rejecting the death and unqualified animal models. ①Water content bf brain in the treatment was less than that in the control group at any time point except the immediate time point, and cerebral edema was relieved [(2.48±0,22)%, (2.32±0.19)%, (2.23±0.36)%, (2.91 ±0.44)%,P 〈 0.05], ②The absolute volume of cerebral infarction in the t
出处
《中国临床康复》
CSCD
北大核心
2006年第38期76-78,共3页
Chinese Journal of Clinical Rehabilitation
基金
辽宁省社会发展基金资助项目(99225003)~~