摘要
运用天冬氨酸转氨酶(AAT)、苹果酸脱氢酶(MDH)以及酯酶(EST)3种同工酶对栽培黄瓜“长春密刺”Cucumissativuscv.Changchunmici(2n=14)与野黄瓜C.hystrix(2n=24)的正反交种间杂种F1(正交:野黄瓜×栽培黄瓜“长春密刺”,反交:栽培黄瓜“长春密刺”×野黄瓜)及其双亲进行鉴定和比较研究。结果表明:正反交种间杂种F1主要表现为双亲酶带的互补,同时还形成4个杂合带(Aat-1-94、Aat-2-104、Mdh-3-102和Est-5-102)。上述3种酶均能准确地鉴定种间杂种的真实性。研究还发现正反交杂种F1的AAT和MDH的酶谱分别在酶带数目和强弱上表现出一定的差异,进一步证实了野黄瓜与栽培黄瓜杂交存在正反交差异。
Electrophoresis of aspartate aminotransferase （AAT）, malate dehydrogenase （MDH） and esterase （EST） were performed to characterize and compare the reciprocal interspecific hybrid plants between cultivated cucumber （Cucumis sativus cv. Changchunmici, 2n=14, CC） and its wild relative C. hystrix （2n=24, HH）, and their parents. The results indicate that the zymograms in the reciprocal F1 were typically expressed by complementary bands from both parents. In addition, four heterodimeric bands （Aat-1-94, Aat-2-104, Mdh-3-102 and Est-5-102） were observed. All the three isozymes investigated could identify the interspecific hybrid plants. The results also reveal the differences of the reciprocal F1s at the number and intensity of the bands in the zymograms of AAT and MDH, which further confirmed the reciprocal differences in the interspecific hybridization between C. hystrix and C. sativus.
出处
《植物分类学报》
CSCD
北大核心
2006年第5期488-493,共6页
Acta Phytotaxonomica Sinica
基金
国家自然科学基金(30470120)
国家"863"计划项目(2004AA241120)
PickleSeedResearchFundofPicklePackerInternational
Inc.~~
关键词
同工酶
黄瓜
种间杂种
isozyme, cucumber, interspecific hybrid.
