摘要
目的:应用有限采样法(Limited Sampling Strategy,LSS)估算肾移植患者口服环孢素后药时曲线下面积(AUC)。方法:12名肾移植患者单剂量口服CsA微乳制剂,荧光免疫偏振法测定各采样时间点CsA的血药浓度,以多元线性逐步回归法建立数学模型。结果:单个血药浓度-时间点预测AUC0-12的回归模型,线性关系较差,2点和3点预测AUC0-12的回归模型较单点预测好(r2>0．9,P<0．05)。其中C2,C6预测AUC0-12的回归模型(AUC0-12=1017．029+3．047 C2+3.121 C6,r2=0．960, P<0．05)线性关系佳且准确性好。结论:以LSS法估算口服CsA微乳制剂AUC0-12准确性好,临床上可用作CsA治疗药物监测的手段。
Objective： To estimate areas under the curve （AUC） of cyclosporin A （CsA） by limited sampling strategy （LSS）. Method： Twelve renal transplant recipients were orally administrated with CsA microemulsion. The concentrations of CsA in whole blood were determined by fluorescence immunoassay. Stepwise multilinear regression analysis was performed to establish a model to es- timate AUC of CsA. Result： The linearity relationship between AUC and single concentration point was not good. 2 or 3 sampling meth- od for AUC of CsA estimation met the predefined criteria （ r2 〉 0.9, P 〈 0.05 ）. The AUC of CsA was best estimated by C2 and C6 （A UC0-12 = 1017. 029 ＋ 3. 047 C2 ＋ 3.121 C6, r2 = 0. 960, P 〈 0.05 ）. Conclusion： A multilinear regression model for estimation AUC of CsA can be developed by LSS method. The method was accuracy and considered desirable for CsA therapeutic drug monitoring.
出处
《中国药师》
CAS
2006年第10期909-911,共3页
China Pharmacist
